It has been illustrated that relaxin hormone in association with estrogen may induce changes in functional body systems following menopausal stage. Therefore, the aim of this study was to determine the relationship between relaxin levels and musculoskeletal injuries in young and menopausal trained women. The present study used descriptive analytical method via retrospective manner. This study involved two determined groups included menopausal trained women (n=13, aged 45-60 years) and young trained women (n=8, aged 18-30 years) that were chose following public recall based on inclusion and exclusion criteria. Then, study measurements regarding blood samples, training level, training history, menopause stage, injuries and musculoskeletal impairments were done. Finally, Stata v12 statistical software was used for data analysis at (p < 0.05). Our results illustrated that relaxin level in menopausal trained women significantly was lower than young trained women (p = 0.003, t19=3.46). Also, our results presented that there was no statistically significant relationship between relaxin and musculoskeletal injuries (p>0.05). Regarding postmenopausal hormone changes, it has been shown that relaxin hormone in relation with estrogen hormone can induce alterations in functions body systems.