One of the issues that has been considered by sports researchers today is the claim of the effect of functional differences in the muscles of the core stability area on the results of kinematic and kinetic variables during the activities of athletes and non-athletes. The aim of this study was to investigate the interaction between lateral core stability area muscles function, ground reaction force and changes in center of mass to pressure in the Medio-lateral direction during gait according to the level of physical activity. Eighteen young men in two groups of 9 athletes and non-athletes participated in this study as subjects. Electrical activity of internal and external oblique muscles and information on the ground reaction force and changes in the center of mass to the presser were recorded during gait. The T-test was used to compare the results of the two groups at p ≤ 0.05. The duration of electrical activity of the external oblique muscle of athletes and non-athletes showed a significant difference (0.005), while no significant difference was observed in the electromechanical delay of the muscles. There was a significant difference between the two groups in the changes in center of mass to pressure of the Medio-lateral direction (0.041). The results of the study confirmed the interactive effect of the lateral core stability muscles and the behavioral pattern of changes in the center of mass to pressure and the ground reaction force in the Medio-lateral direction according to the level of physical activity during gait.